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MAXLISA Vitamin B12 Elisa

INDENTED USE: This kit allows for the determination of B12 concentrations in Human serum, cell culture supernates and other biological fluids.

Product Fearures:

  • Sandwich ELISA method
  • HRP labeled goat anti-human secondary antibody
  • TMB substrate produces blue color and turns yellow after stopping reaction
  • Absorbance measured spectrophotometrically at 450 nm
  • Detection based on OD value compared to standard curve
  • Suitable for serum, cell culture supernates and other biological fluids
  • Assay performed at 37°C with incubation steps

Categories:

  • 96 determinations
  • Wash solution – 20 ml × 1 bottle
  • HRP-Conjugate reagent – 6 ml × 1 bottle
  • Microelisa stripplate – 12 well × 8 strips
  • Sample diluent – 6 ml × 1 bottle
  • Chromogen Solution A – 6 ml × 1 bottle
  • Chromogen Solution B – 6 ml × 1 bottle
  • Stop Solution – 6 ml × 1 bottle
  • Standard (1200 ng/L) – 0.5 ml × 1 bottle
  • Standard diluent – 1.5 ml × 1 bottle
  • Closure plate membrane – 2
  • Sealed bags – 1
  • Assay range: 25 ng/L – 600 ng/L
  • 96 determinations
  • Quantitative detection of Vitamin B12 in human serum, cell culture supernates and other biological fluids
  • Sandwich ELISA method
  • HRP labeled goat anti-human secondary antibody
  • TMB substrate reaction (blue to yellow color change)
  • Absorbance measured at 450 nm

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