MaxLISA HBsAg 3.0 Gen ELISA kit
(Enzyme Linked Immunosorbent Assay)
INTENDED USE: MaxLISA HBsAg 3.0 (Ag) ELISA Test is a one-step enzyme immunoassay diagnostic kit for in vitro qualitative determination of hepatitis B surface antigen (HBsAg) concentration in human serum or plasma (Heparin, Citrate or EDTA). This test is not automated.
Product Fearures:
- Sensitivity: 100%
- Specificity: 99.8 %
- Intra Assay Precision: CV 15% (negative), 4% (weak positive),5% (medium positive)
- Inter Assay Precision: CV 25% (negative), 9% (weak positive),6% (medium positive)
- Cross Reactivity: No false positive results were observed on 5 positive specimens of dengue, HCV, HIV, pylori, syphilis, ANA, HAMA, RF (up to 8400 IU/mL), respectively.
- Interference: No interference was seen with bilirubin (6.7mg/dL), EDTA (1.1 µM), salicylic acid (1.3%), creatinine (147 µM), heparin (1000- U/L), glucose (18 mmol/L)
- Shelf life: 15 months
- Open vial stability: 8 weeks at 2-8°C, or until the expiration date
Categories: Elisa Test Kit
| Ref. No. | Pack Size |
|---|---|
| AVELHBV3-96 | 96 Tests |
- Test Type: Enzyme Linked Immunosorbent Assay (ELISA)
- Application: Qualitative determination of HBsAg in human serum or plasma (Heparin, Citrate or EDTA)
- Assay Principle: Enzyme linked immunosorbent assay (ELISA) with monoclonal anti-HBsAg coated microwells and peroxidase conjugate
- Detection Method: Color development using TMB substrate measured spectrophotometrically
- Specimen: Human serum or plasma
- Incubation: 60 minutes at 37°C
- Washing: Wash wells 5 times with buffer
- Reading Wavelength: 450 nm with reference 620 nm
- Result Interpretation: Based on cut-off value (NCx + 0.2)
- Sensitivity: 100%
- Specificity: 99.8%
